Biotin anti-human CD106 Antibody anti-CD106

Clone: STA (See other available formats)
Regulatory Status: RUO
Workshop: V A013
Other Names: VCAM-1, INCAM-110
Isotype: Mouse IgG1, κ
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Product Citations: publications

STA_Biotin_070507 — TNF-α stimulated HUVEC cells stained with biotinylated STA, followed by Sav-PE

Cat #	Size	Price	Save
305804	100 µg	¥41,360

Description

CD106 is a 110 kD single chain type I glycoprotein also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence³, immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation², ELISA² capture for sCD106, and spatial biology (IBEX)^5,6.
RRID: AB_314560 (BioLegend Cat. No. 305804)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 110 kD
Distribution: Activated endothelial cells, endothelial progenitors, follicular dendritic cells
Function: Leukocyte adhesion, transmigration, costimulation
Ligand/Receptor: VLA-4 (CD49d/CD29)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Carlos T, et al. 1994. Blood 84:2068.
2. Jones E, et al. 1995. Nature 373:539.
Gene ID: 7412 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD106 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD106	STA	FC
Biotin anti-human CD106	STA	FC
PE anti-human CD106	STA	FC,SB
PE/Cyanine5 anti-human CD106	STA	FC
Purified anti-human CD106	STA	FC,IHC-P,ICC,IP,ELISA
TotalSeq™-A0245 anti-human CD106	STA	PG
PE/Cyanine7 anti-human CD106	STA	FC
Brilliant Violet 421™ anti-human CD106	STA	FC
TotalSeq™-C0245 anti-human CD106	STA	PG
TotalSeq™-B0245 anti-human CD106	STA	PG
KIRAVIA Blue 520™ anti-human CD106	STA	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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