Biotin anti-human CD64 Antibody anti-CD64

Clone: 10.1 (See other available formats)
Regulatory Status: RUO
Workshop: VI MA36
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ
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Human peripheral blood monocytes stained with 10.1 FITC

Human peripheral blood monocytes stained with 10.1 FITC

Cat #	Size	Price	Save
305004	100 µg	¥41,360

Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².
RRID: AB_314488 (BioLegend Cat. No. 305004)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?: Yes
How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD64 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
Purified anti-human CD64	10.1	FC,IHC-F,Block
Alexa Fluor® 488 anti-human CD64	10.1	FC
Alexa Fluor® 647 anti-human CD64	10.1	FC
APC anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
Brilliant Violet 421™ anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
APC/Cyanine7 anti-human CD64	10.1	FC
Brilliant Violet 510™ anti-human CD64	10.1	FC
Purified anti-human CD64 (Maxpar® Ready)	10.1	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD64	10.1	FC
Brilliant Violet 605™ anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
PE/Dazzle™ 594 anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
TotalSeq™-A0162 anti-human CD64	10.1	PG
Brilliant Violet 711™ anti-human CD64	10.1	FC
Alexa Fluor® 700 anti-human CD64	10.1	FC
Brilliant Violet 785™ anti-human CD64	10.1	FC
TotalSeq™-C0162 anti-human CD64	10.1	PG
Ultra-LEAF™ Purified anti-human CD64	10.1	FC,IHC-F,Block
TotalSeq™-B0162 anti-human CD64	10.1	PG
TotalSeq™-D0162 anti-human CD64	10.1	PG
GMP PE anti-human CD64	10.1	FC
GMP FITC anti-human CD64	10.1	FC
Brilliant Violet 650™ anti-human CD64	10.1	FC
GMP PE/Dazzle™ 594 anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
GMP APC/Fire™ 750 anti-human CD64	10.1	FC
GMP PE/Cyanine7 anti-human CD64	10.1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 03/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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