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- Clone
- AF6-78 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Igh-6b
- Isotype
- Mouse (BALB/c) IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with biotinylated AF6-78, followed by Sav-PE
This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-Cb and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-Ce can be detected with this antibody.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
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FC - Quality tested
ELISA Detection - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.
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Application References
(PubMed link indicates BioLegend citation) -
- Stall AM, et al. 1984. J. Immunol. 132:787.
- Velardi A, et al. 1984. J. Immunol. 133:2098.
- Product Citations
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- RRID
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AB_315037 (BioLegend Cat. No. 406204)
Antigen Details
- Biology Area
- Immunology
- Gene ID
- 16019 View all products for this Gene ID
- UniProt
- View information about IgMb on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All IgMb Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse IgMb | AF6-78 | FC,ELISA Detection |
| FITC anti-mouse IgMb | AF6-78 | FC |
| PE anti-mouse IgMb | AF6-78 | FC |
| Purified anti-mouse IgMb | AF6-78 | FC,ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse IgMb
C57BL/6 mouse splenocytes stained with biotinylated AF6-78, ... -
FITC anti-mouse IgMb
C57BL/6 mouse splenocytes stained with AF6-78 FITC -
PE anti-mouse IgMb
C57BL/6 mouse splenocytes stained with AF6-78 PE -
Purified anti-mouse IgMb
C57BL/6 mouse splenocytes stained with purified AF6-78, foll...
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