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Biotin anti-rat IFN-γ Antibody

Pricing & Availability
Clone
Poly5188 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Goat Polyclonal Ig
Ave. Rating
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Product Citations
publications
Poly5188_Biotin_IFNgamma_Antibody_1_ELISA_051316
  • Poly5188_Biotin_IFNgamma_Antibody_1_ELISA_051316
  • Poly5188_Biotin_IFNgamma_Antibody_2_WB_051316
    Western blot analysis of 50 ng recombinant human IFN-γ (lane 1), recombinant rat IFN-γ (lane 2), and recombinant mouse IFN-γ (lane 3) using biotin anti-rat IFN-γ (clone Poly5188) antibody followed by HRP Streptavidin secondary antibody.
Cat # Size Price Save
518803 50 µg ¥82,500
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant rat IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1 - 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of rat IFN-γ recombinant protein ranging from 2000 to 31 pg/mL are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated poly5188 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified DB-1 antibody (Cat. No. 507801 or 507808) as the capture antibody and recombinant rat IFN-γ (Cat. No. 580009) as the standard.

Note: For testing rat IFN-γ in serum, plasma or cell culture supernatant, BioLegend's ELISA Legend Max™ Kits (Cat. No. 439007 & 439008) are specially developed and recommended.

Product Citations
  1. Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed
RRID
AB_2561418 (BioLegend Cat. No. 518803)

Antigen Details

Structure
Cytokine, dimer, 40-80 kD (Mammalian)
Distribution
CD8+ and CD4+ T cells, NK cells
Function
Upregulated by IL-2, bFGF, EGF, downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
T cells, NK cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-rat IFN-γ Poly5188 ELISA Detection,WB
Go To Top Version: 3    Revision Date: 06/01/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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