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- Regulatory Status
- RUO
- Other Names
- Adult folate-binding protein (FBP), Folate receptor 1, Folate receptor, adult KB cells FBP, Ovarian tumor-associated antigen MOv18
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- Product Citations
- publications
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Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid-BSA conjugate) in a dose-dependent manner. The ED50 for this effect is 0.5-3.0 ng/mL. -
Stability Testing for Recombinant Human FOLR1. Biotinylated Recombinant Human FOLR1 was aliquoted in PBS, pH 7.0 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to FA-BSA (folic acid-BSA conjugate) in a dose-dependent manner. The ED50 for this effect is 0.5-3.0 ng/mL
| Cat # | Size | Price | Save |
|---|---|---|---|
| 565504 | 25 µg | ¥70,650 | |
| 565506 | 100 µg | ¥176,120 |
FOLR1, also known as folate receptor alpha, is a glycosylphosphatidylinositol (GPI)-anchored cell-surface glycoprotein encoded by the FOLR1 gene. FOLR1 binds with high affinity to folic acid and its derivatives and mediates cellular processes, including cell division, proliferation, and tissue growth via signaling cascades and components of the folate cycle. Each FOLR only binds one folate and brings the folate into cells via endocytosis of the FOLR-folate complex. Human FOLR1 shares 83% amino acid sequence homology with mouse and rat FOLR1. FOLR1, the most extensively studied isoform, has limited tissue expression but is overexpressed in several tumors of epithelial origin including ovarian cancer, non-small cell lung adenocarcinoma, breast cancer, renal cancer and endometrial cancer. The overexpression of FOLR1 is often associated with increased cancer progression and poor patient prognosis. It plays a critical role during embryogenesis.
Product DetailsProduct Details
- Source
- Human FOLR1, amino acid (Arg25-Met233) (Accession: # P15328) with a C-terminal 8His and Avi tag, was expressed in CHO cells.
- Molecular Mass
- The 246 amino acid recombinant protein has a predicted molecular mass of approximately 28.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 35-45 kD and 35-40 kD respectively as determined by SDS-PAGE. The predicted N-terminal amino acid is Arg.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at - 20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid BSA conjugate). The EC50 of this effect is 0.5 – 3.0 ng/mL.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- GPI-anchored N-glycosylated
- Distribution
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Plasma membrane, GPI-anchored, ER membrane, endosome, limited expression on the apical surfaces of the epithelial cells of normal lung, breast, thyroid, parathyroid, and kidney tissues
- Function
- Folate receptor (FOLR1) binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. It is required for embryonic development and cell proliferation.
- Ligand/Receptor
- Folate and folic acid derivatives
- Bioactivity
- Biotinylated Recombinant Human FOLR1 binds to immobilized FA-BSA (folic acid BSA conjugate). The EC50 of this effect is 0.5 – 3.0 ng/mL.
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Signal Transduction
- Molecular Family
- Carrier Proteins
- Antigen References
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- Luhrs CA & Slomiany BL. 1989. J Biol Chem. 264:21446-9.
- Sierra EEP & Goldman ID. 1999. Semin Oncol. 26:11-23.
- Cheung A, et al. 2018. Clin Cancer Res. 24:5098-111.
- Cheung A, et al. 2016. Oncotarget. 7:52553–74.
- Kelemen LE. 2006. Int J Cancer. 119:243-50.
- Spannuth WA, et al. 2010. Expert Opin Biol Ther. 10:431-437.
- Kelemen LE, et al. 2004. Cancer Causes Control. 15:1085-93.
- Kelemen LE, et al. 2008. Cancer Res. 68:2498-506.
- Liu JJ, et al. 2010. Adv Genet. 71:79-121.
- Kotsopoulos J, et al. 2010. Int J Cancer. 126:2191-2198.
- Nawab FN and Kipreos ET. 2022. Trends Endocrinol Metab. 33:159-174.
- Piedrahita JA, et al. 1999. Nat. Genet. 23:228.
- Gene ID
- 2348 View all products for this Gene ID
- UniProt
- View information about FOLR1 on UniProt.org
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