Brilliant Violet 421™ anti-human CCX-CKR (CCRL1) Antibody, CCX-CKR (CCRL1), 13E11

Clone: 13E11 (See other available formats)
Regulatory Status: RUO
Other Names: ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR11, CKR-11, CCXCKR
Isotype: Mouse IgG2b, κ
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13E11_BV421_CCS-CKR_Antibody_053024 — Human CCX-CKR-transfected bovine coronary artery endothelial cells were stained with anti-human CCX-CKR (CCRL1) (clone 13E11) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
362109	25 tests	¥42,460
362110	100 tests	¥89,540

Description

Human CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length CCX-CKR cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates¹, induction of CCX-CKR internalization¹ and immunofluorescence¹. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.
RRID: AB_3106250 (BioLegend Cat. No. 362109)
AB_3106250 (BioLegend Cat. No. 362110)

Antigen Details

Structure: G-protein-coupled seven transmembrane receptor, 40 kD.
Distribution: Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.
Function: C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.
Interaction: β-arrestin.
Ligand/Receptor: CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.
Cell Type: Dendritic cells, Epithelial cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169.
2. Comerford I, et al. 2010. Blood. 116:4130.
Gene ID: 51554 View all products for this Gene ID
UniProt: View information about CCX-CKR on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CCX-CKR (CCRL1) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CCX-CKR (CCRL1)	13E11	FC,ICC,IP,Agonist
PE anti-human CCX-CKR (CCRL1)	13E11	FC
APC anti-human CCX-CKR (CCRL1)	13E11	FC
PE/Cyanine7 anti-human CCX-CKR (CCRL1)	13E11	FC
Brilliant Violet 421™ anti-human CCX-CKR (CCRL1)	13E11	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/30/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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