Brilliant Violet 421 anti-human CD360 IL-21R Antibody anti-CD360

Clone: 2G1-K12 (See other available formats)
Regulatory Status: RUO
Other Names: IL-21 Receptor, novel interleukin receptor (NILR)
Isotype: Mouse IgG1, κ
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Product Citations: publications

2G1-K12_BV421_IL21R_Antibody_FC_1_101512

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
347809	25 tests	¥47,300

Description

The human interleukin 21 receptor (IL-21R), is a single pass type I membrane protein and a member of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibits the greatest extracellular homology to the IL-2R beta subunit, i.e., contains one copy of the WSXWS-containing cytokine-binding domain. Intracellular domains of IL-21R include the Box 1 and Box 2 elements which are similar to the IL-9R intracellular region. Upon binding IL-21, the IL-21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Stat signaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is a potent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly the inflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IL-21R transfected Ba/F3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2561651 (BioLegend Cat. No. 347809)

Antigen Details

Structure: Single pass type I membrane protein, extracellular WSXWS-containing cytokine-binding domain, intracellular Box1 and Box2 domains
Distribution: B cells, various levels on NK cells and T cells
Ligand/Receptor: IL-21
Cell Type: B cells, NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Parish-Novak J, et al. 2000. Nature 408:57.
2. Ozaki K, et al. 2000. Proc Natl. Acad. Sci. USA. 97:11439.
3. Dumoutier L, et al. 2000. Proc Natl. Acad. Sci. USA. 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
6. de Torero D, et al. 2006. Blood 107:3708.
7. Davis ID, et al.2007. Clin Cancer Res. 13:6926.
8. Nurieva R, et al. 2007. Nature 448:4416.
Gene ID: 50615 View all products for this Gene ID
UniProt: View information about CD360 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Th17 Biology

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD360 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD360 (IL-21R)	2G1-K12	FC
PE anti-human CD360 (IL-21R)	2G1-K12	FC
APC anti-human CD360 (IL-21R)	2G1-K12	FC
Brilliant Violet 421™ anti-human CD360 (IL-21R)	2G1-K12	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 02/07/2013

For Research Use Only. Not for diagnostic or therapeutic use.

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