Brilliant Violet 421™ anti-mouse CD25 Antibody, CD25, A18246A

Clone: A18246A (See other available formats)
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2a, κ
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Product Citations: publications

A18246A_Brilliant-Violet-421_-CD25_Antibody_061523 — C57BL/6 mouse splenocytes were stained with anti-mouse CD4 FITC and anti-mouse CD25 (clone A18246A) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
113705	50 µg	¥51,700

Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3068095 (BioLegend Cat. No. 113705)

Antigen Details

Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor
Ligand/Receptor: IL-2
Antigen References: Taniguchi T, et al. 1993. Cell. 73:5-8.

Waldmann TA. 1991. J Biol Chem. 266:2681-4.

Read S, et al. 2000. J Exp Med. 192:295-302.

Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID: 16184 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD25 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD25	A18246A	FC
PE anti-mouse CD25	A18246A	FC
Brilliant Violet 421™ anti-mouse CD25	A18246A	FC
Brilliant Violet 605™ anti-mouse CD25	A18246A	FC
APC anti-mouse CD25	A18246A	FC
PE/Cyanine7 anti-mouse CD25 Antibody	A18246A	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/15/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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