- Clone
- CB225 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TLR-2, mTLR2, Ly105
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD282, also known as TLR2, is an 89 kD, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular region and one toll/IL-1R (TIR) domain in the intracellular region. TLR2 is mainly expressed on monocytes, macrophages, dendritic cells, granulocytes, and endothelial and epithelial cells. TLR2 associates with TLR1 and TLR6, and mediates the innate immune response against several components of Gram-positive bacteria, such as peptidoglycan, lipoteichoic acid (LTA), lipoarabinomanan, and lipoproteins as well as different LPS from certain Gram-negative bacteria, yeast, spirochetes, and fungi.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TLR2-transfected Ba/F3 cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
1. Shibata T, et al. 2012. Int. Immunol. 24:613. (FC)
- RRID
-
AB_3106173 (BioLegend Cat. No. 148605)
Antigen Details
- Structure
- 89 kD type I transmembrane protein with 14 leucine-rich repeats (LRR) on its extracellular region and one toll/IL-1R (TIR) domain on its intracellular region.
- Distribution
-
Monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, epithelial cells.
- Function
- Activates innate host defense against a diverse set of pathogens.
- Interaction
- TLR1, TLR6.
- Ligand/Receptor
- Microbial lipoproteins, peptidoglycans, lipoteichoic acid.
- Cell Type
- Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
-
1. Du B, et al. 2013. J. Biol. Chem. 288:15131.
2. Zivkovic A, et al. 2011. J. Immunol. 186:1608.
3. Okun E, et al. 2010. J. Neurochem. 114:462.
4. Kim S, et al. 2009. Nature. 457:102. - Gene ID
- 24088 View all products for this Gene ID
- UniProt
- View information about CD282 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD282 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD282 (TLR2) | CB225 | FC |
PE anti-mouse CD282 (TLR2) | CB225 | FC |
Brilliant Violet 421™ anti-mouse CD282 (TLR2) | CB225 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD282 (TLR2)
Mouse TLR2-transfected Ba/F3 cells were stained with purifie... -
PE anti-mouse CD282 (TLR2)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w... -
Brilliant Violet 421™ anti-mouse CD282 (TLR2)
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w...
Follow Us