- Clone
- MIH63 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD-1 homolog, Dies1, B7-H5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein that is composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and natural killer cells (NK). It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants due to its ability to preferentially suppress CD4+ T cell-mediated immunity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse VISTA transfected J558 cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Product Citations
-
- RRID
-
AB_2728192 (BioLegend Cat. No. 150212)
Antigen Details
- Structure
- Type I transmembrane protein, member of the CD28 family.
- Distribution
-
Macrophages, dendritic cells, neutrophils, natural killer cells, and naive and activated T cells.
- Function
- Negative regulator of the immune response.
- Cell Type
- Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Antigen References
-
1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp. Med. 208:577.
3. Flies DB, et al. 2014. J. Clin. Invest. 124:1966. - Gene ID
- 74048 View all products for this Gene ID
- UniProt
- View information about VISTA on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All VISTA Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse VISTA (PD-1H) | MIH63 | FC |
PE anti-mouse VISTA (PD-1H) | MIH63 | FC |
APC anti-mouse VISTA (PD-1H) | MIH63 | FC |
Biotin anti-mouse VISTA (PD-1H) | MIH63 | FC |
Brilliant Violet 421™ anti-mouse VISTA (PD-1H) | MIH63 | FC |
PerCP/Cyanine5.5 anti-mouse VISTA (PD-1H) | MIH63 | FC |
PE/Dazzle™ 594 anti-mouse VISTA (PD-1H) | MIH63 | FC |
PE/Cyanine7 anti-mouse VISTA (PD-1H) | MIH63 | FC |
TotalSeq™-A0915 anti-mouse VISTA (PD-1H) | MIH63 | PG |
TotalSeq™-C0915 anti-mouse VISTA (PD-1H) | MIH63 | PG |
TotalSeq™-B0915 anti-mouse VISTA (PD-1H) | MIH63 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with purified PD-1H (... -
PE anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC and PD-1... -
APC anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-... -
Biotin anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC and PD-1... -
Brilliant Violet 421™ anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 75... -
PerCP/Cyanine5.5 anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 75... -
PE/Dazzle™ 594 anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 75... -
PE/Cyanine7 anti-mouse VISTA (PD-1H)
C57BL/6 mouse splenocytes were stained with CD8 APC/Fire™ 75... -
TotalSeq™-A0915 anti-mouse VISTA (PD-1H)
-
TotalSeq™-C0915 anti-mouse VISTA (PD-1H)
-
TotalSeq™-B0915 anti-mouse VISTA (PD-1H)
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