- Clone
- Poly4054 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-P - Reported in the literature, not verified in house
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescence staining. For IF staining, the suggested dilution of this reagent is 1:20 - 1:50. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_10900808 (BioLegend Cat. No. 405414)
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Goat anti-rat IgG (minimal x-reactivity)
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FITC Goat anti-rat IgG (minimal x-reactivity)
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HRP Goat anti-rat IgG (minimal x-reactivity)
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PE Goat anti-rat IgG (minimal x-reactivity)
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Purified Goat anti-rat IgG (minimal x-reactivity)
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PE/Cyanine7 Goat anti-rat IgG (minimal x-reactivity)
C57BL/c mouse splenocytes stained with purified rat anti-mou... -
Cyanine3 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity)
293E cells were stained with purified anti-Histone H3-Phosph... -
Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity)
Human frozen tonsil section was fixed with 4% paraformaldehy... -
Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity)
NIH/3T3 mouse fibroblasts were fixed with 1% paraformaldehyd... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity)
NIH/3T3 mouse fibroblast cell line was fixed with 1% parafor... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PerCP/Cyanine5.5 Goat anti-rat IgG (minimal x-reactivity)
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APC/Fire™ 750 Goat anti-rat IgG (minimal x-reactivity)
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Brilliant Violet 605™ Goat anti-rat IgG (minimal x-reactivity)
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PE/Dazzle™ 594 Goat anti-rat IgG (minimal x-reactivity)
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Biotin Goat anti-rat IgG (minimal x-reactivity)
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