Brilliant Violet 421™ anti-human CD177 Antibody, CD177, MEM-166

Clone: MEM-166 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype: Mouse IgG1, κ
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Product Citations: publications

MEM-166_BV421_CD177_Antibody_1_110724-2 — Human peripheral blood cells were stained with anti-human CD177 (clone MEM-166) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right).

Compare all formats See Brilliant Violet 421™ spectral data

Cat #	Size	Price	Save
315817	25 tests	¥42,460
315818	100 tests	¥89,540

Description

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human granulocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting⁵, and immunofluorescence⁴.

Antigen Details

Structure: uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution: Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)
Function: Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification: Glycosylated
Cell Type: Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.
Gene ID: 57126 View all products for this Gene ID
UniProt: View information about CD177 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD177 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD177	MEM-166	FC,ICC,IP,WB
FITC anti-human CD177	MEM-166	FC
PE anti-human CD177	MEM-166	FC
APC anti-human CD177	MEM-166	FC
APC/Cyanine7 anti-human CD177	MEM-166	FC
TotalSeq™-A0382 anti-human CD177	MEM-166	PG
TotalSeq™-C0382 anti-human CD177	MEM-166	PG
TotalSeq™-B0382 anti-human CD177	MEM-166	PG
Brilliant Violet 421™ anti-human CD177 Antibody	MEM-166	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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