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Brilliant Violet 421™ anti-human IL12RB2 Antibody

Pricing & Availability
Clone
S16020B (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 receptor subunit beta-2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
S16020B_BV421_IL12RB2_Antibody_051922
Human peripheral blood lymphocytes were activated for 3 days with anti-human CD3 and anti-human CD28, and then stained with anti-human IL12RB2 (clone S16020B) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • S16020B_BV421_IL12RB2_Antibody_051922
    Human peripheral blood lymphocytes were activated for 3 days with anti-human CD3 and anti-human CD28, and then stained with anti-human IL12RB2 (clone S16020B) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Save
394211 25 tests ¥42,460
394212 100 tests ¥89,540
Description

IL12RB2 is a type I transmembrane protein that foms an heterodimer with IL12RB1 to produce the high affinity IL-12 receptor complex. IL12RB2 is expressed by activated T cells, TH1 cells, NK cells, B cells and plasma cells. Variants of this gene are associated with predisposition to various immune-related diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL12RB2 transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
RRID
AB_2922619 (BioLegend Cat. No. 394211)
AB_2922619 (BioLegend Cat. No. 394212)

Antigen Details

Structure
Type I transmembrane protein, 3 isoforms, foms an heterodimer with IL12RB1
Distribution

Activated T cells, TH1 cells, NK cells, B cells,  plasma cells

Function
Role in TH1 differentiation
Ligand/Receptor
IL-12
Cell Type
B cells, NK cells, Plasma cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. LaMere SA, et al. 2017. J Immunol. 199:3158
  2. Prigione I, et al. 2016. Immunobiology. 221:291
  3. de Paus RA, et al. 2013. Mol Immunol. 56:380
  4. Airoldi I, et al. 2008. Blood. 112:750
Gene ID
3595 View all products for this Gene ID
UniProt
View information about IL12RB2 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 05/19/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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