- Clone
- ACK2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- c-KIT, Stem Cell Factor Receptor (SCFR)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine IL-3 dependent mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3
ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). -
Application References
(PubMed link indicates BioLegend citation) -
- Yoshinaga K, et al. 1991. Development 113:689.
- Broudy VC, et al. 1996. Blood 88:75.
- Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.
- Product Citations
-
- RRID
-
AB_2562011 (BioLegend Cat. No. 135119)
Antigen Details
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5. - Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD117 (c-kit) | ACK2 | FC |
PE anti-mouse CD117 (c-kit) | ACK2 | FC |
APC anti-mouse CD117 (c-kit) | ACK2 | FC |
PE/Cyanine7 anti-mouse CD117 (c-kit) | ACK2 | FC |
Ultra-LEAF™ Purified anti-mouse CD117 (c-kit) | ACK2 | FC,Block,Depletion |
Alexa Fluor® 488 anti-mouse CD117 (c-kit) | ACK2 | FC |
FITC anti-mouse CD117 (c-kit) | ACK2 | FC |
Brilliant Violet 510™ anti-mouse CD117 (c-kit) | ACK2 | FC |
Brilliant Violet 605™ anti-mouse CD117 (c-kit) | ACK2 | FC |
Brilliant Violet 421™ anti-mouse CD117 (c-kit) | ACK2 | FC |
Brilliant Violet 650™ anti-mouse CD117 (c-kit) | ACK2 | FC |
PE/Dazzle™ 594 anti-mouse CD117 (c-kit) | ACK2 | FC |
Biotin anti-mouse CD117 (c-kit) | ACK2 | FC |
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) | ACK2 | FC |
APC/Cyanine7 anti-mouse CD117 (c-kit) | ACK2 | FC |
Brilliant Violet 785™ anti-mouse CD117 (c-kit) | ACK2 | FC |
APC/Fire™ 750 anti-mouse CD117 (c-kit) | ACK2 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with ACK2 PE and CD1... -
PE anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with ACK2 PE and CD1... -
APC anti-mouse CD117 (c-kit)
C57BL/6 bone marrow cells stained with ACK2 APC and CD11b (M... C57BL/6 bone marrow cells stained with rat IgG2b APC isotype... -
PE/Cyanine7 anti-mouse CD117 (c-kit)
Bone marrow cells from C57BL/6 mice stained with CD11b (clon... -
Ultra-LEAF™ Purified anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with ACK2 PE and CD1... -
Alexa Fluor® 488 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
FITC anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Brilliant Violet 510™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Brilliant Violet 605™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Brilliant Violet 421™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Brilliant Violet 650™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PE/Dazzle™ 594 anti-mouse CD117 (c-kit)
C57BL/6 bone marrow cells were stained with CD11b APC and CD... -
Biotin anti-mouse CD117 (c-kit)
C57BL/6 bone marrow cells were stained with CD11b APC and bi... -
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
APC/Cyanine7 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 785™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
APC/Fire™ 750 anti-mouse CD117 (c-kit)
C57BL/6 bone marrow cells were stained with anti-mouse CD11b...
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