- Clone
- Sa14-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LPS receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Product Citations
-
- RRID
-
AB_2564129 (BioLegend Cat. No. 123323)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
macrophages, dendritic cells, kupffer cells, hepatocytes
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089. - Gene ID
- 12475 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Related FAQs
Other Formats
View All CD14 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD14 | Sa14-2 | FC |
Biotin anti-mouse CD14 | Sa14-2 | FC |
FITC anti-mouse CD14 | Sa14-2 | FC |
PE anti-mouse CD14 | Sa14-2 | FC |
APC anti-mouse CD14 | Sa14-2 | FC |
PerCP/Cyanine5.5 anti-mouse CD14 | Sa14-2 | FC |
PE/Cyanine7 anti-mouse CD14 | Sa14-2 | FC |
APC/Cyanine7 anti-mouse CD14 | Sa14-2 | FC |
Purified anti-mouse CD14 (Maxpar® Ready) | Sa14-2 | FC,CyTOF® |
Brilliant Violet 510™ anti-mouse CD14 | Sa14-2 | FC |
Purified anti-mouse CD14 | Sa14-2 | WB, IF, FC |
Brilliant Violet 421™ anti-mouse CD14 | Sa14-2 | FC |
Alexa Fluor® 647 anti-mouse CD14 | Sa14-2 | FC |
PE/Dazzle™ 594 anti-mouse CD14 | Sa14-2 | FC |
APC/Fire™ 750 anti-mouse CD14 | Sa14-2 | FC |
TotalSeq™-A0424 anti-mouse CD14 | Sa14-2 | PG |
TotalSeq™-C0424 anti-mouse CD14 | Sa14-2 | PG |
TotalSeq™-B0424 anti-mouse CD14 | Sa14-2 | PG |
Brilliant Violet 605™ anti-mouse CD14 | Sa14-2 | FC |
Brilliant Violet 785™ anti-mouse CD14 Antibody | Sa14-2 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD14
BALB/c peritoneal macrophages stained with Sa14-2 purified, ... -
Biotin anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
FITC anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
PE anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
APC anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
PerCP/Cyanine5.5 anti-mouse CD14
C57BL/6 peritoneal macrophage cells stained with Sa14-2 PerC... -
PE/Cyanine7 anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages s... -
APC/Cyanine7 anti-mouse CD14
Thioglycolate-elicited Balb/c mouse peritoneal macrophages w... -
Purified anti-mouse CD14 (Maxpar® Ready)
MC/9 (top) or J774A.1 (bottom) mouse cell lines stained with... -
Brilliant Violet 510™ anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
Purified anti-mouse CD14
-
Brilliant Violet 421™ anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
Alexa Fluor® 647 anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
PE/Dazzle™ 594 anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
APC/Fire™ 750 anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
TotalSeq™-A0424 anti-mouse CD14
-
TotalSeq™-C0424 anti-mouse CD14
-
TotalSeq™-B0424 anti-mouse CD14
-
Brilliant Violet 605™ anti-mouse CD14
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ... -
Brilliant Violet 785™ anti-mouse CD14 Antibody
Thioglycollate-elicited BALB/c mouse peritoneal macrophages ...
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