- Clone
- 9E2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKp46, NCR1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9E2_BV650_CD335_Antibody_FC_1_082013.jpg 9E2_BV650_CD335_Antibody_FC_1_082013.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E2_BV650_CD335_Antibody_FC_1_082013.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD56 FITC and CD335 (clone 9E2) Brilliant Violet 650™ (top) or mouse IgG1, κ Brilliant Violet 650™ isotype control (bottom). -
CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NKp46-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Clone 9E2 has been shown to block NK activation through NKp46.6
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2562442 (BioLegend Cat. No. 331927)
Antigen Details
- Structure
- Type I membrane glycoprotein (46 kD)
- Distribution
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Expressed on resting and activated NK cells
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
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1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656. - Gene ID
- 9437 View all products for this Gene ID
- UniProt
- View information about CD335 on UniProt.org
Related FAQs
Other Formats
View All CD335 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with purified 9E2... -
Biotin anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 biotin, ... -
PE anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with CD56 (HCD56)... -
Alexa Fluor® 647 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 Alexa fl... -
Pacific Blue™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 Pacific ... -
Brilliant Violet 421™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 AP... -
PerCP/Cyanine5.5 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 FI... -
APC anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 PE... -
PE/Cyanine7 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 FI... -
FITC anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 AP... -
Brilliant Violet 510™ anti-human CD335 (NKp46)
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Brilliant Violet 605™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 AP... -
Brilliant Violet 650™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 FI... -
PE/Dazzle™ 594 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 AP... -
Alexa Fluor® 700 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 PE... -
Brilliant Violet 711™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 FI... -
APC/Fire™ 750 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 FI... -
Alexa Fluor® 488 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 AP... -
TotalSeq™-B0101 anti-human CD335 (NKp46)
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TotalSeq™-C0101 anti-human CD335 (NKp46)
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TotalSeq™-A0101 anti-human CD335 (NKp46)
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Brilliant Violet 785™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 PE... -
Ultra-LEAF™ Purified anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with purified 9E2... -
APC/Cyanine7 anti-human CD335 (NKp46) Antibody
Human peripheral blood lymphocytes were stained with CD56 (c... -
PE/Cyanine5 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with anti-human C... -
TotalSeq™-D0101 anti-human CD335 (NKp46)
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PE/Cyanine7 anti-human CD335
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™)
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