- Clone
- 11F6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mki67, Ki67, Ki-67, MIB-1, KIA
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![11F6_Brilliant Violet 711_ki-67_Antibody_032023 11F6_Brilliant Violet 711_ki-67_Antibody_032023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/11F6_Brilliant-Violet-711_ki-67_Antibody_032023-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol at -20°C for an hour and stained with anti-mouse/human Ki-67 (clone 11F6) Brilliant Violet 711™ (filled histogram) or rat IgG2b, κ Brilliant Violet 711™ isotype control (open histogram). -
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol at - 20°C for an hour and stained with Helix NP™ Green and anti-mouse/human Ki-67 (clone 11F6) Brilliant Violet 711™ (left), or rat IgG2b, κ Brilliant Violet 711™ isotype control (right). -
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol at -20°C for an hour and stained with anti-mouse CD25 APC and anti-mouse/human Ki-67 (clone 11F6) Brilliant Violet 711™ (left), or rat IgG2b, κ Brilliant Violet 711™ isotype control (right).
The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- RRID
-
AB_2936670 (BioLegend Cat. No. 151227)
Antigen Details
- Structure
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
- Distribution
-
Nucleus and chromosomes.
- Function
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Ki-67 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse/human Ki-67 | 11F6 | IHC-F,ICC |
Alexa Fluor® 488 anti-mouse/human Ki-67 | 11F6 | ICFC,ICC,IHC-F |
Alexa Fluor® 647 anti-mouse/human Ki-67 | 11F6 | ICFC,ICC,IHC-F |
Brilliant Violet 421™ anti-mouse/human Ki-67 | 11F6 | ICFC,IHC-F |
PE anti-mouse/human Ki-67 | 11F6 | ICFC |
Alexa Fluor® 594 anti-mouse/human Ki-67 | 11F6 | IHC-F |
FITC anti-mouse/human Ki-67 | 11F6 | ICFC |
Brilliant Violet 650™ anti-mouse/human Ki-67 | 11F6 | ICFC |
PE/Cyanine7 anti-mouse/human Ki-67 | 11F6 | ICFC |
PE/Dazzle™ 594 anti-mouse/human Ki-67 | 11F6 | ICFC |
PerCP/Cyanine5.5 anti-mouse/human Ki-67 | 11F6 | ICFC |
Pacific Blue™ anti-mouse/human Ki-67 | 11F6 | ICFC |
Brilliant Violet 711™ anti-mouse/human Ki-67 | 11F6 | ICFC |
Brilliant Violet 510™ anti-mouse/human Ki-67 | 11F6 | ICFC |
APC/Cyanine7 anti-mouse/human Ki-67 | 11F6 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse/human Ki-67
TE-71 cells were fixed with 1% paraformaldehyde (PFA) for te... C57BL/6 mouse frozen intestine section was fixed with 4% par... -
Alexa Fluor® 488 anti-mouse/human Ki-67
TE‐71 cells were fixed with 1% paraformaldehyde (PFA) for te... C57BL/6 mouse frozen intestine section was fixed with 4% par... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Alexa Fluor® 647 anti-mouse/human Ki-67
TE-71 cells were fixed with 1% paraformaldehyde (PFA) for te... C57BL/6 mouse frozen intestine section was fixed with 4% par... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Brilliant Violet 421™ anti-mouse/human Ki-67
C57BL/6 frozen intestine section was fixed with 4% paraforma... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
PE anti-mouse/human Ki-67
ConA/IL-2 treated C57BL/6 mouse splenocytes were fixed and p... -
Alexa Fluor® 594 anti-mouse/human Ki-67
C57BL/6 mouse frozen intestine section was fixed with 4% par... -
FITC anti-mouse/human Ki-67
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes we... -
Brilliant Violet 650™ anti-mouse/human Ki-67
C57BL/6J splenocytes treated with ConA and IL-2 for 2 days w... -
PE/Cyanine7 anti-mouse/human Ki-67
C57BL/6J splenocytes treated with ConA and IL-2 for 3 days w... -
PE/Dazzle™ 594 anti-mouse/human Ki-67
C57BL/6J splenocytes treated with ConA and IL-2 for 3 days w... -
PerCP/Cyanine5.5 anti-mouse/human Ki-67
C57BL/6J splenocytes treated with ConA and IL-2 for 3 days w... -
Pacific Blue™ anti-mouse/human Ki-67
C57BL/6J splenocytes treated with ConA and IL-2 for 3 days w... -
Brilliant Violet 711™ anti-mouse/human Ki-67
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Brilliant Violet 510™ anti-mouse/human Ki-67
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
APC/Cyanine7 anti-mouse/human Ki-67
Con A and IL-2 stimulated (2 days) C57BL/6 mouse splenocytes... Con A and IL-2 stimulated (2 days) C57BL/6 mouse splenocytes...
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