- Clone
- RTK2758 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
-
Application References
(PubMed link indicates BioLegend citation) -
- Nishimoto H, et al. 2005. Blood 106:4241.
- Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
- Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
- RRID
-
AB_3097190 (BioLegend Cat. No. 402607)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
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Related FAQs
Other Formats
View All ReagentsCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Rat IgG2a, κ Isotype Ctrl
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Biotin Rat IgG2a, κ Isotype Ctrl
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FITC Rat IgG2a, κ Isotype Ctrl
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PE Rat IgG2a, κ Isotype Ctrl
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PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl
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Purified Rat IgG2a, κ Isotype Ctrl
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PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl
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APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
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Pacific Blue™ Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl
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PerCP Rat IgG2a, κ Isotype Ctrl
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PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl
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Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl
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PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl
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Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl
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GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl
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APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl
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KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl
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APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl
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Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl
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Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl
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Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl
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TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl
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Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl
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Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl
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PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl
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Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl
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PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl
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Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl
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Spark UV™ 387 Rat IgG2a, κ Isotype Ctrl
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PE/Fire™ 744 Rat IgG2a, κ Isotype Ctrl
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Spark PLUS UV395™ Rat IgG2a, κ Isotype Ctrl
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