- Clone
- DREG-56 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V S056
- Other Names
- L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
304829 | 25 tests | ¥45,980 | |
304830 | 100 tests | ¥91,960 |
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Concentrated supernatant from PMA-activated human peripheral blood leukocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block)
- Jutila M, et al. 2002. J. Immunol. 169:1768. (WB)
- Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB)
- Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed
- Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed
- Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
- Product Citations
-
- RRID
-
AB_2629555 (BioLegend Cat. No. 304829)
AB_2629555 (BioLegend Cat. No. 304830)
Antigen Details
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
- Kishimoto T, et al. 1991. Blood 78:805.
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 APC -
FITC anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 FITC -
PE anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PE -
PE/Cyanine5 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PE/C... -
Purified anti-human CD62L
Human peripheral blood lymphocytes stained with purified DRE... -
APC/Cyanine7 anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Alexa Fluor® 488 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Alex... -
Alexa Fluor® 647 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG56 Alexa... -
Alexa Fluor® 700 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Alex... -
PE/Cyanine7 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PE/C... -
PerCP/Cyanine5.5 anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Pacific Blue™ anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Paci... -
Brilliant Violet 421™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Brilliant Violet 785™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Brilliant Violet 650™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
PE/Dazzle™ 594 anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Brilliant Violet 605™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Purified anti-human CD62L (Maxpar® Ready)
Human Jurkat T cells (top) and human NALM-6 pre-B cells (bot... -
APC/Fire™ 750 anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Brilliant Violet 510™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
TotalSeq™-A0147 anti-human CD62L
-
TotalSeq™-B0147 anti-human CD62L
-
TotalSeq™-C0147 anti-human CD62L
-
Ultra-LEAF™ Purified anti-human CD62L
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ... -
Brilliant Violet 711™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
Spark NIR™ 685 anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (... -
TotalSeq™-D0147 anti-human CD62L
-
APC/Fire™ 810 anti-human CD62L
Human peripheral lymphocytes were stained with anti-human 62... -
FITC anti-human CD62L
Typical results from Human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD62L
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Fire™ 806 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD62L
Typical result from human peripheral lymphocytes stained eit... -
GMP FITC anti-human CD62L
Typical results from Human peripheral blood lymphocytes stai... -
Biotin anti-human CD62L
Human peripheral blood mononuclear cells were stained with a... -
Spark Blue™ 574 anti-human CD62L (Flexi-Fluor™)
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