- Clone
- Poly4064 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Donkey Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly4064_Cyanine3_IgG_Antibody_050520 Poly4064_Cyanine3_IgG_Antibody_050520](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly4064_Cyanine3_IgG_Antibody_050520.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 1 µg/mL of purified anti-Giantin (clone Poly19087) and followed by Cyanine3 Donkey anti-rabbit IgG (clone Poly4064) antibody (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
Product DetailsProduct Details
- Verified Reactivity
- Rabbit
- Antibody Type
- Polyclonal
- Host Species
- Donkey
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Cyanine3 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
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It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
- Additional Product Notes
-
BioLegend is in the process of converting the name Cy3 to Cyanine3. The dye molecule remains the same, so you should expect the same quality and performance from our Cyanine3 products. Please contact Technical Service if you have any questions.
- Product Citations
-
- RRID
-
AB_893532 (BioLegend Cat. No. 406402)
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Rabbit IgG Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
HRP Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ELISA,IHC,WB |
Cyanine3 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC |
FITC Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC,FC |
Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC,FC |
DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC,FC |
DyLight™ 649 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC,FC |
Alexa Fluor® 647 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | FC |
Alexa Fluor® 488 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | FC |
PE Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | FC |
Brilliant Violet 510™ Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | FC |
Alexa Fluor® 594 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC |
Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity) | Poly4064 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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HRP Donkey anti-rabbit IgG (minimal x-reactivity)
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Cyanine3 Donkey anti-rabbit IgG (minimal x-reactivity)
HeLa cells were fixed with ice cold methanol for six minutes... -
FITC Donkey anti-rabbit IgG (minimal x-reactivity)
Hela cells were stained with anti-GRASP65 (Poly6210) and sec... -
Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity)
293E cells were stained by purified anti-Tubulin-gamma (clon... -
DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity)
293T cells were stained with anti-Tubulin-γ and secondarily ... -
DyLight™ 649 Donkey anti-rabbit IgG (minimal x-reactivity)
-
Alexa Fluor® 647 Donkey anti-rabbit IgG (minimal x-reactivity)
C57/BL6 splenocytes were stained with NK-1.1 Brilliant Viole... -
Alexa Fluor® 488 Donkey anti-rabbit IgG (minimal x-reactivity)
C57BL/6 splenocytes were stained with NK-1.1 Brilliant Viole... -
PE Donkey anti-rabbit IgG (minimal x-reactivity)
C57BL/6 splenocytes were stained with NK-1.1 APC and purifie... -
Brilliant Violet 510™ Donkey anti-rabbit IgG (minimal x-reactivity)
C57BL/6 splenocytes were stained with NK-1.1 APC and purifie... -
Alexa Fluor® 594 Donkey anti-rabbit IgG (minimal x-reactivity)
HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 ... -
Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity)
HeLa cells were fixed with 1% paraformaldehyde for ten minut...
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