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DAB Substrate Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Substrate Buffer
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Product Citations
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Cat # Size Price Save
926605 15 mL ¥11,880
926606 1000 mL ¥92,180
Description

In the presence of peroxidase enzyme, DAB will produce a brown precipitate that is insoluble in alcohol. This product is the substrate buffer for use with DAB chromogen (Cat. Nos. 926506 and 926507).

Product Details
Technical data sheet

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

IHC
Recommended Usage

Each lot of this product is quality tested by immunohistochemistry.

Prepare the DAB solution by combining components as described in the following table.

Buffer     DAB Chromogen
5 mL   +   0.2 mL
25 mL +   1.0 mL
50 mL +   2.0 mL

Application Notes

Protocol:
1. Add the DAB chromogen to the DAB substrate buffer as described above
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 minutes at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background

Product Citations
  1. Ratz-Mitchem ML, et al. 2023. Mamm Genome. 572:34. PubMed

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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