Pricing & Availability
Technical data sheet
- Regulatory Status
- RUO
- Other Names
- Chromogen Substrate Solution
- Previously
-
Covance Catalog# SIG-31049
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Product Details
- Formulation
- Chromogen Substrate Buffer.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC
- Recommended Usage
-
Protocol:
1. Add the DAB chromogen to the DAB substrate buffer as described in the table below
Buffer DAB Chromogen*
5 mL + 0.2 mL
25 mL + 1.0 mL
50 mL + 2.0 mL
*Cat Nos: 926501 & 926503.
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background - Application Notes
-
BioLegend’s DAB Substrate Buffer with Stabilizer consists of stable buffer. The DAB Substrate Buffer with Stabilizer in combination with DAB chromogen reacts with peroxidase to produce brown staining.
Antigen Details
- Gene ID
- NA
Follow Us