Direct-Blot HRP anti-ERK1/2 Phospho Thr202/Tyr204 Antibody anti-ERK1/2

Clone: 4B11B69 (See other available formats)
Regulatory Status: RUO
Other Names: P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype: Mouse IgG2b, κ
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4B11B69_ERK1slash2Phospho_WB_Image_062116 — 15 µg of total protein extract from Jurkat cells treated with PMA was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69). Proteins were visualized using chemiluminescence detection.

Cat #	Size	Price	Save
675506	25 µL	¥22,220
675505	100 µL	¥74,800

Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
RRID: AB_2566700 (BioLegend Cat. No. 675506)
AB_2566700 (BioLegend Cat. No. 675505)

Antigen Details

Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Cytoplasm. Nucleus.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
Gene ID: 5594 View all products for this Gene ID
UniProt: View information about ERK1/2 on UniProt.org

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Other Formats

View All ERK1/2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-ERK1/2 Phospho (Thr202/Tyr204)	4B11B69	WB,ICC
Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204)	4B11B69	WB
Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204)	4B11B69	ICFC
Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)	4B11B69	ICFC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 06/21/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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