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Direct-Blot™ HRP anti-RNA Polymerase II RPB3 Antibody

Pricing & Availability
Clone
1Y26 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase II sub-unit 3, RPB3, POLR2C
Isotype
Mouse IgG1
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Product Citations
publications
1Y26_DBHRP_RNAPolymeraseIIRPB3_Antibody_031519_updated.png
Total cell lysates (15 µg total protein) from HeLa and S. cerevisiae (yeast) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Direct-Blot™ anti-RPB3 Antibody, clone 1Y29, overnight at 4°C. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • 1Y26_DBHRP_RNAPolymeraseIIRPB3_Antibody_031519_updated.png
    Total cell lysates (15 µg total protein) from HeLa and S. cerevisiae (yeast) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Direct-Blot™ anti-RPB3 Antibody, clone 1Y29, overnight at 4°C. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Save
665005 100 µL ¥74,800
Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
RRID
AB_2566702 (BioLegend Cat. No. 665005)

Antigen Details

Structure
RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
854791 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB3 on UniProt.org
Go To Top Version: 5    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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