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- Regulatory Status
- RUO
- Other Names
- BLys, TNFSF13B, B cell activating factor
- Ave. Rating
- Submit a Review
- Product Citations
- publications
BAFF (known as B Lymphocyte Stimulator) is a member of the Tumor Necrosis Factor Ligand Superfamily. The extracellular domain of BAFF shows high homology with the proliferation-inducing ligand (APRIL protein). BAFF acts mainly as a soluble trimer and binds to three receptors with decreasing affinity in the following order: BAFFR > TACI > BCMA. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, SLE, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin’s lymphoma (NHL).
BioLegend’s ELISA MAX™ Deluxe Set is designed for the accurate quantification of natural and recombinant human BAFF in serum, plasma, and cell culture supernatant.
Kit Contents
- Kit Contents
-
- Human BAFF ELISA MAX™ Capture Antibody (200X)
- Human BAFF ELISA MAX™ Detection Antibody (200X)
- Human BAFF Standard Lyophilized
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer B (5X)
- Assay Diluent B (5X)
- Assay Diluent D
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 10.1 ± 7.1 pg/mL
- Standard Range
- 31.25 - 2000 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Epithelial cells, Lymphocytes, Monocytes, Neutrophils, Plasma cells, T cells
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
- Molecular Family
- Tumor Suppressors
- Gene ID
- NA
- UniProt
- View information about BAFF on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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