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- Regulatory Status
- RUO
- Other Names
- Granulocyte colony stimulating factor, CSF-3, CSFG, MGI-IG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Mouse Granulocyte-Colony Stimulating Factor (G-CSF), also known as mouse CSF-3, is a growth factor and pleiotropic cytokine that influences cells from the bone marrow to differentiate, proliferate and activate the neutrophilic granulocyte lineage (Neutrophils). Mouse G-CSF is produced primarily by monocytes and macrophages upon activation and is measured and tested for differentiation studies. Mouse G-CSF belongs to the IL-6 superfamily and is a polypeptide chain containing 179 amino acids and has a molecular weight of 19 kD. It is naturally produced by the body and is also available as an injection. Human and mouse G-CSF proteins share 76% sequence identity at the amino acid level.
Product DetailsKit Contents
- Kit Contents
-
- Mouse G-CSF Capture Antibody (200X)
- Mouse G-CSF Detection Antibody (200X)
- Mouse G-CSF Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer B (5X)
- Assay Diluent B (5X)
- Blocking Buffer A (2X)
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 3.8 - 8.6 pg/mL
- Standard Range
- 12.5 - 800 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- NA
- UniProt
- View information about G-CSF on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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