FITC anti-human CD107b LAMP-2 Antibody anti-CD107b

Clone: H4B4 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: LAMPb, LGP110
Isotype: Mouse IgG1, κ
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Product Citations: publications

H4B4_FITC_032312 — Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with CD107b (clone H4B4) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
354305	25 tests	¥23,540
354306	100 tests	¥47,520

Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines. LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Adult human adherent spleen cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli² and immunofluorescent staining of neutrophils^2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
RRID: AB_11203905 (BioLegend Cat. No. 354305)
AB_11203905 (BioLegend Cat. No. 354306)

Antigen Details

Structure: LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution: Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines
Function: Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type: B cells, Lymphocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538.
Gene ID: 3920 View all products for this Gene ID
UniProt: View information about CD107b on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD107b Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD107b (LAMP-2)	H4B4	ICFC
PE anti-human CD107b (LAMP-2)	H4B4	ICFC
Purified anti-human CD107b (LAMP-2)	H4B4	ICFC,IHC-P,ICC,WB
Alexa Fluor® 594 anti-human CD107b (LAMP-2)	H4B4	IHC-P
Alexa Fluor® 647 anti-human CD107b (LAMP-2)	H4B4	ICFC,IHC-P
Brilliant Violet 421™ anti-human CD107b (LAMP-2)	H4B4	ICFC,IHC-P
Brilliant Violet 785™ anti-human CD107b (LAMP-2)	H4B4	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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