Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- HI100
- Workshop
- IV N906
- Isotype
- Mouse IgG2b, κ
![HI100_FITC_CD45RA_ASR_011117 HI100_FITC_CD45RA_ASR_011117](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HI100_FITC_CD45RA_ASR_011117.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood lymphocytes stained either with HI100 FITC used at 5 µL/test (solid line) or with an isotype control (dotted line).
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
Suggested for Flow Cytometry
- Disclaimer
-
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
Antigen Details
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 APC -
Biotin anti-human CD45RA
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 FITC -
PE anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... -
PE/Cyanine5 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 PE/Cya... -
Purified anti-human CD45RA
Human peripheral blood platelets stained with purified HI100... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 488 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa ... -
Alexa Fluor® 647 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... Human paraffin-embedded tonsil tissue slices were prepared w... -
Pacific Blue™ anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Pacifi... -
Alexa Fluor® 700 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa ... -
PerCP/Cyanine5.5 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
PE/Cyanine7 anti-human CD45RA
Human peripheral blood lymphocytes were stained with HI100 P... -
APC/Cyanine7 anti-human CD45RA
Human peripheral blood lymphocytes were stained CD45RA (clon... -
Brilliant Violet 421™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... Human paraffin-embedded tonsil tissue slices were prepared w... -
Brilliant Violet 570™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 605™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 650™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 711™ anti-human CD45RA
Human peripheral blood lymphocytes stained with CD45RA (clon... -
Brilliant Violet 785™ anti-human CD45RA
Human peripheral blood lymphocytes stained with CD45RA (clon... -
Brilliant Violet 510™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Purified anti-human CD45RA (Maxpar® Ready)
Human PBMCs stained with 149Sm-anti-CD45RO (UCHL1) and 143Nd... -
PE/Dazzle™ 594 anti-human CD45RA
Human peripheral blood granulocytes were stained with CD45RA... -
APC/Fire™ 750 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
PerCP anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... -
FITC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0063 anti-human CD45RA
-
Alexa Fluor® 594 anti-human CD45RA
Human paraffin-embedded tonsil tissue slices were prepared w... -
TotalSeq™-B0063 anti-human CD45RA
-
TotalSeq™-C0063 anti-human CD45RA
-
Brilliant Violet 750™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Spark NIR™ 685 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
APC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Fire™ 640 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD45RA Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark YG™ 581 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0063 anti-human CD45RA
-
Spark Violet™ 423 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... IHC staining of Spark Violet™ 423 anti-human CD45RA (clone H... -
GMP FITC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-Bn0063 anti-human CD45RA
-
Spark Blue™ 550 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Dazzle™ 594 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
Spark PLUS UV™ 395 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu...
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