FITC anti-human EGFR Antibody, EGFR, A21043C

Clone: A21043C (See other available formats)
Regulatory Status: RUO
Other Names: Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Isotype: Mouse IgG1, κ
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Product Citations: publications

A21043C_FITC_EGFR_Antibody_3 — Human cervical cancer cell line HeLa was stained with anti-human EGFR (clone A21043C) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
386305	25 tests	¥37,620
386306	100 tests	¥84,040

Description

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of EGFR, aminoacids 25-138
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)

Antigen Details

Structure: Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution: Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
Function: Controls cell growth and differentiation
Ligand/Receptor: Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: CD Molecules, Growth Factors
Antigen References: da Cunha Santos G, et al. 2011. Annu Rev Pathol. 6:49-69.

Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522-7.

Mano M and Humblet Y. 2008. Nat Clin Pract Oncol. 5:415-25.

Pao W and Chmielecki J. 2010. Nat Rev Cancer. 10:760-74.
Gene ID: 1956 View all products for this Gene ID
UniProt: View information about EGFR on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All EGFR Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human EGFR	A21043C	FC
PE anti-human EGFR	A21043C	FC
FITC anti-human EGFR	A21043C	FC
APC anti-human EGFR	A21043C	FC
Spark UV™ 387 anti-human EGFR Antibody	A21043C	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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