FITC anti-human HLA-DR Antibody anti-HLA-DR

Clone: Tü36 (See other available formats)
Regulatory Status: RUO
Other Names: MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
Isotype: Mouse IgG2b, κ
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Product Citations: publications

Tu36_FITC_HLA-DR_Antibody_FC_022014 — Human peripheral blood lymphocytes were stained with purified HLA-DR (clone Tü36) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).

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Cat #	Size	Price	Save
361603	25 tests	¥22,220
361604	100 tests	¥55,660

Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus, Cat, Dog, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (of relevant formats) includes Western blotting⁴, immunoprecipitation⁴, and in vitro blocking⁵. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
RRID: AB_2563164 (BioLegend Cat. No. 361603)
AB_2563164 (BioLegend Cat. No. 361604)

Antigen Details

Structure: Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution: B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5.
Gene ID: 3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID
UniProt: View information about HLA-DR on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All HLA-DR Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human HLA-DR	Tü36	FC,IP,WB
PerCP/Cyanine5.5 anti-human HLA-DR	Tü36	FC
APC anti-human HLA-DR	Tü36	FC
FITC anti-human HLA-DR	Tü36	FC
PE anti-human HLA-DR	Tü36	FC
PE/Cyanine7 anti-human HLA-DR	Tü36	FC
APC/Fire™ 750 anti-human HLA-DR	Tü36	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 11/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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