FITC anti-human/mouse ZAP-70 Antibody anti-ZAP-70

Clone: 1E7.2 (See other available formats)
Regulatory Status: RUO
Other Names: Zeta chain associated protein kinase, SYK related tyrosine kinase
Isotype: Mouse IgG1, κ
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Human peripheral blood lymphocytes intracellularly stained with 1E7.2 FITC and CD3 APC

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Cat #	Size	Price	Save
313406	100 tests	¥45,320

Description

ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: GST-fusion protein corresponding to residues 282-307 of human ZAP-70
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation², Western blotting².

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
RRID: AB_2241894 (BioLegend Cat. No. 313406)

Antigen Details

Structure: Protein tyrosine kinase, contains two SH2 domains, 70 kD
Distribution: T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL)
Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect
Ligand/Receptor: Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc
Modification: Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity)
Cell Type: Leukemia, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Chan AC, et al. 1992. Cell 71:649.
2. Chan AC, et al. 1994. Science 264:1559.
3. Arpaia E, et al. 1994. Cell 76:947.
Gene ID: 22637 View all products for this Gene ID 7353 View all products for this Gene ID
UniProt: View information about ZAP-70 on UniProt.org

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Other Formats

View All ZAP-70 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse ZAP-70	1E7.2	ICFC,WB,IP
PE anti-human/mouse ZAP-70	1E7.2	ICFC
FITC anti-human/mouse ZAP-70	1E7.2	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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