FITC anti-mouse CD206 (MMR) Recombinant Antibody

Clone: QA17A35 (See other available formats)
Regulatory Status: RUO
Other Names: Macrophage mannose receptor, Mannose receptor, MCR1
Isotype: Rat IgG1, κ
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QA17A35_FITC_CD206_Antibody_011024 — Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with anti-mouse/human CD11b (clone M1/70) APC. Cells were then fixed, permeabilized, and intracellularly stained with anti-mouse CD206 (MMR) recombinant (clone QA17A35) FITC (left) or rat IgG1, κ FITC isotype control (right).

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
162507	25 µg	¥39,600
162508	100 µg	¥88,000

Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This recombinant antibody clone is derived from clone C068C2.
RRID: AB_3097515 (BioLegend Cat. No. 162507)
AB_3097515 (BioLegend Cat. No. 162508)

Antigen Details

Structure: Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
Function: Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine
Antigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Kitamura T. et al. 2018. Front Immunol. 8:2004
4. Laoui D. et al. 2011. Int. J. Dev. Biol. 55: 861 - 867
Gene ID: 17533 View all products for this Gene ID
UniProt: View information about CD206 on UniProt.org

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Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All CD206 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD206 (MMR) Recombinant Antibody	QA17A35	ICFC
PE anti-mouse CD206 (MMR) Recombinant Antibody	QA17A35	ICFC
APC anti-mouse CD206 (MMR) Recombinant Antibody	QA17A35	ICFC
FITC anti-mouse CD206 (MMR) Recombinant Antibody	QA17A35	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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