FITC anti-mouse CD40 Antibody anti-CD40

Clone: HM40-3 (See other available formats)
Regulatory Status: RUO
Other Names: Bp50, TNFRSF5
Isotype: Armenian Hamster IgM
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HM40-3_FITC_090507 — C57BL/6 mouse splenocytes stained with HM40-3 FITC

Compare all formats See FITC spectral data

Cat #	Size	Price	Save
102905	50 µg	¥14,780
102906	500 µg	¥50,910

Description

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34⁺ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: (BALB/c x NZB)F₁ mouse-derived lymphoma WEHI 231
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding¹, stimulation of mouse B cell and dendritic cell proliferation^2-6,9, and immunohistochemistry⁷ of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102908).
RRID: AB_312949 (BioLegend Cat. No. 102905)
AB_312949 (BioLegend Cat. No. 102906)

Antigen Details

Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34⁺ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor: CD40L (CD154)
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
Gene ID: 21939 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD40 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD40	HM40-3	FC
Purified anti-mouse CD40	HM40-3	FC,Block,Costim,IHC-F
Alexa Fluor® 488 anti-mouse CD40	HM40-3	FC
Alexa Fluor® 647 anti-mouse CD40	HM40-3	FC
Ultra-LEAF™ Purified anti-mouse CD40	HM40-3	FC,Block,Costim,IHC-F

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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