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- Clone
- OX-38 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- L3T4, T4
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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LOU rat splenocytes stained with OX-38 FITC
CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat thymocyte glycoproteins
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
The OX-38 antibody has been shown to bind the same CD4 epitope as the W3/25 antibody, and a different epitope than the OX-35 antibody. Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections and blocking function1.
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Application References
(PubMed link indicates BioLegend citation) -
- Pettigrew GJ, et al. 1998. J. Immunol. 161:1292.
- RRID
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AB_1227552 (BioLegend Cat. No. 203405)
Antigen Details
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Pettigrew GJ, et al. 1998. J. Immunol. 161:1292.
- Gene ID
- 24932 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All CD4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-rat CD4 (domain 1) | OX-38 | FC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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FITC anti-rat CD4 (domain 1)
LOU rat splenocytes stained with OX-38 FITC
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