- Clone
- M5E2 (See other available formats)
- Workshop
- III 329
- Other Names
- LPS receptor
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M5E2_GMP_PE-Dazzle594_CD14_081523 M5E2_GMP_PE-Dazzle594_CD14_081523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M5E2_GMP_PE-Dazzle594_CD14_081523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood monocytes stained either with M5E2 PE/Dazzle™ 594 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.
Product DetailsProduct Details
- Reactivity
- Human,Cynomolgus,Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 200.0 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
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Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Disclaimer
-
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434. - Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Other Formats
View All CD14 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD14
Human peripheral blood monocytes stained with M5E2 APC -
FITC anti-human CD14
Human peripheral blood monocytes stained with M5E2 FITC -
PE anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE -
Purified anti-human CD14
Human peripheral blood monocytes stained with M5E2 APC -
PE/Cyanine7 anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE/Cyanin... -
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
Ultra-LEAF™ Purified anti-human CD14
Human peripheral blood monocytes were stained with Ultra-LEA... -
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes stained with clone M5E2 Pac... -
APC/Cyanine7 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
PerCP/Cyanine5.5 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Biotin anti-human CD14
Human peripheral blood monocytes stained with biotinylated M... -
Brilliant Violet 421™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 570™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 605™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 650™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 711™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 785™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 510™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Purified anti-human CD14 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 160Gd-an... -
PerCP anti-human CD14
Human peripheral blood monocytes were stained with PerCP CD1... -
FITC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Dazzle™ 594 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Pacific Blue™ anti-human CD14
Typical results from human peripheral blood monocytes staine... -
APC/Fire™ 750 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
APC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
TotalSeq™-A0081 anti-human CD14
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TotalSeq™-B0081 anti-human CD14
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TotalSeq™-C0081 anti-human CD14
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PE anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Cyanine5 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
TotalSeq™-D0081 anti-human CD14
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APC/Fire™ 750 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP FITC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Cyanine7 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP APC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP PE anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Dazzle™ 594 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP Pacific Blue™ anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP APC/Fire™ 750 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PerCP/Cyanine5.5 anti-human CD14
Figure Legend: Typical results from human peripheral blood m... -
Spark Violet™ 500 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
GMP PE/Dazzle™ 594 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
APC/Fire™ 810 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
PE/Fire™ 700 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma...
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