- Clone
- WM15 (See other available formats)
- Workshop
- IV M44
- Other Names
- Aminopeptidase N, APN, gp150
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![WM15_GMP_PerCPCyanine5-5_CD13_111323 WM15_GMP_PerCPCyanine5-5_CD13_111323](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/WM15_GMP_PerCPCyanine5-5_CD13_111323.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood granulocytes stained either with WM15 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.
Product DetailsProduct Details
- Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cotton-topped Tamarin
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Saiki I, et al. 1993. Int J Cancer. 54:137. (Block)
- Rosenzwajg M, et al. 2000. Blood 95:453. (Block)
- Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
- Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)
- Disclaimer
-
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
- Distribution
-
Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset
- Function
- Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
- Ligand/Receptor
- Coronavirus receptor
- Cell Type
- Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Shipp M, et al. 1993. Blood 82:1052.
2. Larsen S, et al. 1996. J. Exp. Med. 184:183. - Gene ID
- 290 View all products for this Gene ID
- UniProt
- View information about CD13 on UniProt.org
Related FAQs
Other Formats
View All CD13 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD13
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE anti-human CD13
Human peripheral blood granulocytes stained with WM15 PE -
Purified anti-human CD13
Human peripheral blood granulocytes stained with purified WM... -
Brilliant Violet 421™ anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (... -
APC/Cyanine7 anti-human CD13
Human peripheral blood granulocytes stained with CD13 (clone... -
PE/Cyanine7 anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (... -
PerCP/Cyanine5.5 anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (... -
Purified anti-human CD13 (Maxpar® Ready)
Human PBMCs stained with 152Sm anti-CD13 (WM15) and 169Tm an... -
PE/Dazzle™ 594 anti-human CD13
Human peripheral granulocytes were stained with CD13 (clone ... -
PE anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
Brilliant Violet 711™ anti-human CD13
Human peripheral blood granulocytes stained with CD13 (clone... -
Ultra-LEAF™ Purified anti-human CD13
Human peripheral blood granulocytes stained with Ultra-LEAF™... -
Brilliant Violet 785™ anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (... -
Brilliant Violet 605™ anti-human CD13
Human peripheral blood granulocytes were stained with CD13 (... -
TotalSeq™-A0364 anti-human CD13
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TotalSeq™-B0364 anti-human CD13
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TotalSeq™-C0364 anti-human CD13
-
PE/Cyanine7 anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
TotalSeq™-D0364 anti-human CD13
-
APC anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
PE/Dazzle™ 594 anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
PerCP/Cyanine5.5 anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
GMP APC anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
GMP PE anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
PE/Fire™ 810 anti-human CD13
Human peripheral blood granulocytes were stained with anti-h... -
GMP PE/Cyanine7 anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
GMP PE/Dazzle™ 594 anti-human CD13
Typical results from human peripheral blood granulocytes sta... -
GMP PerCP/Cyanine5.5 anti-human CD13
Typical results from human peripheral blood granulocytes sta...
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