HRP anti-beta-Amyloid, 1-40 Antibody anti-beta-Amyloid, x-40

Clone: 11A50-B10 (See other available formats)
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9146-02
Covance Catalog# SIG-39146
Isotype: Mouse IgG1, κ
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Product Citations: publications

11A50-B10_HRP_betaAmyloid_1-40_1_091019 — IHC staining of HRP anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer’s disease human brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

Cat #	Size	Price	Save
805407	200 µL	¥139,260

Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. For storage of this antibody when diluted below a concentration of 50 µg/mL, a carrier protein such as 2% bovine serum albumin is required.
Application: IHC-P - Quality tested
Dot Blot, ELISA, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.
RRID: AB_2564979 (BioLegend Cat. No. 805407)

Antigen Details

Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351 View all products for this Gene ID
UniProt: View information about beta-Amyloid 1-40 on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All β-Amyloid, 1-40 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-β-Amyloid, 1-40	11A50-B10	ELISA,WB,IHC,IP
HRP anti-β-Amyloid, 1-40	11A50-B10	IHC-P,Dot Blot,ELISA,IP
Purified anti-β-Amyloid, 1-40	11A50-B10	IHC-P,Dot Blot,ELISA

Certificate of Analysis

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Go To Top Version: 6 Revision Date: 02/13/2024

For Research Use Only. Not for diagnostic or therapeutic use.

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