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HRP anti-DJ-1 (PARK7) Antibody

Pricing & Availability
Clone
A16125E (See other available formats)
Regulatory Status
RUO
Other Names
Protein DJ-1, oncogene, DJ1, PARK7, Parkinson disease protein 7, Parkinsonism-Associated Deglycase, HEL-S-67p, epididymis secretory sperm binding protein Li 67p, GATD2, Protein Deglycase DJ-1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A16125E_HRP_DJ-1_Antibody_1_WB_050318
Western blot of HRP anti-DJ-1 (PARK7) antibody (clone A16125E). Lane 1: Molecular weight marker; Lane 2: 20 µg of recombinant human DJ-1 (PARK7) protein (Cat. No. 843401); Lane 3: 20 µg of normal human brain lysate; Lane 4: 20 µg of Parkinson’s disease brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16125E_HRP_DJ-1_Antibody_1_WB_050318
    Western blot of HRP anti-DJ-1 (PARK7) antibody (clone A16125E). Lane 1: Molecular weight marker; Lane 2: 20 µg of recombinant human DJ-1 (PARK7) protein (Cat. No. 843401); Lane 3: 20 µg of normal human brain lysate; Lane 4: 20 µg of Parkinson’s disease brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16125E_HRP_DJ-1_Antibody_2_ICH-P_050318
    IHC staining of HRP anti-DJ-1 (PARK7) antibody (clone A16125E) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
851504 100 µg ¥69,740
Description

DJ-1 (PARK7) belongs to the peptidase C56 family of proteins and is expressed in almost all human cells and tissues. DJ-1 is a multi-functional protein associated with mitochondrial function, mitophagy, and male fertility. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease. Therefore, the protein has an important role in cell protection against oxidative stress and cell death. DJ-1 is also associated with prostate cancer, and specific mutations of the protein are linked to autosomal recessive, early onset Parkinson’s disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human DJ-1
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734642 (BioLegend Cat. No. 851504)

Antigen Details

Structure
Human DJ-1 is a 189 amino acid protein with an apparent molecular weight of 20 kD. The protein consists of 7 β-strands and 7 α-helices, and exits as a dimer.
Distribution

Tissue distribution: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in brain and placenta.
Cellular distribution: Mitochondria, nucleus, cytosol, plasma membrane, and extracellular.

Function
DJ-1 functions as an oxidative stress sensor and redox-sensitive chaperone.
Interaction
PINK1, Parkin, EFCAB6/DJBP, OTUD7B, BBS1, HIPK1, CLCF1 and MTERF.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Mitochondrial Markers
Antigen References
  1. Saito Y. 2014. J. Clin. Biochem. Nutr. 54(3):138-44. PubMed
  2. Ariga H, et al. 2013. Oxid. Med. Cell Longev. 2013:683920. PubMed
  3. Bonifati V, et al. 2003. Science (5604): 256–9. PubMed
Gene ID
11315 View all products for this Gene ID
UniProt
View information about DJ-1 on UniProt.org

Other Formats

View All DJ-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-DJ-1 (PARK7) A16125E WB,Direct ELISA,IHC-P
HRP anti-DJ-1 (PARK7) A16125E WB,IHC-P
Go To Top Version: 1    Revision Date: 05/04/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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