HRP anti-Sortilin Antibody, Sortilin, W16078A

Clone: W16078A (See other available formats)
Regulatory Status: RUO
Other Names: 100 kD NT receptor
Isotype: Rat IgG2a, κ
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Product Citations: publications

Western blot of HRP anti-Sortilin antibody (clone A16078A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 0.2 µg/ml of the primary antibody for 1 hour at room temperature. Enhanced chemiluminescence was used as the detection system.

Cat #	Size	Price	Save
852103	25 µg	¥32,340
852104	100 µg	¥68,420

Description

Sortilin was originally purified from human brain tissue and was identified as a new lipoprotein receptor, which was later identified as a multifunctional sorting receptor of the VPS10P-domain receptor family. Although Sortilin is predominately expressed in neurons of the central and the peripheral nervous systems, it is also expressed in metabolic tissues including liver. Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Sortilin is critical for lipid metabolism, and it has been implicated in lipid disorders. Sortilin is also an APOE receptor implicated in Alzheimer disease.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human SORT1 recombinant protein (327-638 a.a.) expressed in E. coli.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 2 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2801199 (BioLegend Cat. No. 852103)
AB_2801199 (BioLegend Cat. No. 852104)

Antigen Details

Structure

Sortilin is an 831 amino acid protein with a molecular mass of 95 kD.

Distribution

Tissue distribution: Sortilin is expressed in numerous tissues including brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis.

Cellular distribution: Plamsa membrane, cytosol, endosome, endoplasmic reticulum, lysosome, and golgi apparatus.

Function

Sortilin functions as a cell surface receptor and mediates intracellular trafficking of diverse endogenous or exogenous protein substrates between the trans-Golgi network and plasma membrane compartments.

Interaction

Sortilin interacts with p75 Neurotrophin Receptor and is localized together with the golgi marker GM130 and the early endosome marker EEA1.

Ligand/Receptor

Sortilin functions as lipoprotein receptor for neurotrophins and other growth factors.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors

Molecular Family

Golgi Markers

Antigen References

Skeldal S, et al. 2012. J. Biol. Chem. 287:43798-809.
Herda S, et al. 2012. Immunity. 37:854-66.
Gao A, et al. 2017. DNA Cell Biol. 36:1050-1061.
Carlo AS. 2013. Prion. 7:378-82.

Gene ID

6272 View all products for this Gene ID

UniProt

View information about Sortilin on UniProt.org

Documentation

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Other Formats

View All Sortilin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Sortilin	W16078A	WB,IHC-P
HRP anti-Sortilin	W16078A	WB,IHC-P

Certificate of Analysis

Go To Top Version: 3 Revision Date: 12/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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