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ELISA MAX™ Deluxe Set Human IL-2

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-2 ELISA Kit
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Product Citations
publications
human il-2_122109
  • human il-2_122109
Cat # Size Price Save
431804 5 plates ¥67,100
431815 10 Plates ¥128,920
431816 20 Plates ¥206,360
Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Human IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human IL-2 ELISA MAX™ Capture Antibody (200X)
  • Human IL-2 ELISA MAX™ Detection Antibody (200X)
  • Human IL-2 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution F mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Poirier N, et al. 2010. Sci Transl Med. 2:17ra10. (Deluxe kit) PubMed
  2. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  3. Sturner KH, et al. 2014. J Immunol. 193:4439. PubMed
  4. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
Product Citations
  1. Yang L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  2. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  3. Sim MJW, et al. 2022. Elife. 11: . PubMed
  4. Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed
  5. Labanieh L, et al. 2022. Cell. 185:1745. PubMed
  6. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  7. Shi T, et al. 2023. J Clin Invest. . PubMed
  8. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  9. Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
  10. Tello‐Lafoz M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16370-w. PubMed
  11. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  12. Stürner K, et al. 2014. J Immunol. 193:4439. PubMed
  13. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  14. Waldrip ZJ, et al. 2021. J Biol Chem. 297:101209. PubMed
  15. Gigliotti CL, et al. 2022. Front Immunol. 13:915963. PubMed
  16. Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed
  17. Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
  18. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  19. Albring A, et al. 2014. Pharmacol Biochem Behav. 16:126. PubMed
  20. Redkiewicz P, et al. 2012. Plant Biotechnol J. 10:806. PubMed
  21. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  22. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  23. Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
  24. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  25. Lee C, et al. 2020. Biomaterials. 262:120338. PubMed
  26. Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
  27. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2 SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2 HPO4 , 0.2 g KH2 PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Sources
T cells
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 07/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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