KIRAVIA Blue 520™ anti-mouse CD135 Antibody, CD135, A2F10

Clone: A2F10 (See other available formats)
Regulatory Status: RUO
Other Names: Flk-2, Flt3, Ly-72
Isotype: Rat IgG2a, κ
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Product Citations: publications

A2F10_KB520_CD135_Antibody_062923 — C57BL/6 bone marrow cells stained with anti-mouse CD45R/B220 PE and anti-mouse CD135 (clone A2F10) KIRAVIA Blue 520™ (left) or rat IgG2a, κ KIRAVIA Blue 520™ isotype control (right).

Compare all formats See KIRAVIA Blue 520™ spectral data

Cat #	Size	Price	Save
135323	25 µg	¥36,300
135324	100 µg	¥82,500

Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Flt3 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.

KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3068160 (BioLegend Cat. No. 135323)
AB_3068160 (BioLegend Cat. No. 135324)

Antigen Details

Structure: A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution: Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
Function: Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor: Flk-2/FLT3 ligand
Cell Type: B cells, Hematopoietic stem and progenitors
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478
Gene ID: 14255 View all products for this Gene ID
UniProt: View information about CD135 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Myeloid-Derived Suppressor Cells

Related FAQs

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Other Formats

View All CD135 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD135	A2F10	FC
Biotin anti-mouse CD135	A2F10	FC
APC anti-mouse CD135	A2F10	FC
PE/Cyanine5 anti-mouse CD135	A2F10	FC
Brilliant Violet 421™ anti-mouse CD135	A2F10	FC
TotalSeq™-A0098 anti-mouse CD135	A2F10	PG
TotalSeq™-B0098 anti-mouse CD135	A2F10	PG
TotalSeq™-C0098 anti-mouse CD135	A2F10	PG
KIRAVIA Blue 520™ anti-mouse CD135	A2F10	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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