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LEAF™ Purified anti-human IFN-γ Antibody

Pricing & Availability
Clone
Poly5400 (See other available formats)
Regulatory Status
RUO
Other Names
Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5400_LEAF_IFN-gamma_Antibody_101821
Recombinant human IFN-γ (Cat. No. 570206) (black circles) induces cytotoxicity with recombinant human TNF-α (Cat. No. 570106) on HT-29 cells. LEAF™ purified anti-human IFN-γ antibody (Poly5400) (purple squares) reverses the effect of recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg /mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 1-5 μg/mL.
  • Poly5400_LEAF_IFN-gamma_Antibody_101821
    Recombinant human IFN-γ (Cat. No. 570206) (black circles) induces cytotoxicity with recombinant human TNF-α (Cat. No. 570106) on HT-29 cells. LEAF™ purified anti-human IFN-γ antibody (Poly5400) (purple squares) reverses the effect of recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg /mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 1-5 μg/mL.
Cat # Size Price Save
540003 100 µg ¥96,800
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the cytotoxicity induced by recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg/mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells. ND50 range: 1-5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904434 (BioLegend Cat. No. 540003)

Antigen Details

Structure
Cytokine; dimer
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Antigen References
  1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
  2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
  3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
  4. Gray P, et al. 1987. Lymphokines 13:151.
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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