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- Regulatory Status
- RUO
- Other Names
- Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human IL-6 Standard ranging from 0.47 pg/mL to 30 pg/mL.
IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, and CTL differentiation factor, etc.) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth and it induces nerve cells differentiation. In hepatocytes, it induces acute phase reactants. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis.
The BioLegend LEGEND MAX™ High Sensitivity Human IL-6 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with rat monoclonal anti-human IL-6 antibody. The detection antibody is a biotinylated rat monoclonal anti-human IL-6 antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IL-6. This kit is specifically designed for the accurate quantitation of IL-6 in serum, plasma, cell culture supernatant, and urine. This kit is analytically validated with ready-to-use reagents.
Kit Contents
- Kit Contents
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- High Sensitivity Human IL-6 Pre-coated 96-well Strip Microplate
- Human IL-6 Detection Antibody
- Human IL-6 Lyophilized Standard
- Streptavidin-Polymer HRP
- Assay Buffer A
- 20X Wash Buffer
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 0.143 ± 0.033 pg/mL
- Standard Range
- 0.47 – 30 pg/mL
- Materials Not Included
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- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 μL to 1000 μL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate shaker
- Polypropylene vials
Antigen Details
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3569 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
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We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
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The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
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In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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