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- Regulatory Status
- RUO
- Other Names
- VEGF, IL-10, EGF, TNF-α, IL-2, IL-5, GM-CSF, CXCL-10 (IP-10), IFN-γ, IL-1β, IL-6, CXCL-9(MIG), TGF-β, TB Panel, TB Biomarker Panel
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- Submit a Review
- Product Citations
- publications
Tuberculosis (TB), a respiratory disease caused by the bacterium Mycobacterium tuberculosis, has emerged as a global threat over the years. In 2022, TB was the world’s second largest cause of death by a single infectious pathogen, after COVID-19. According to estimates, nearly a quarter of the world’s population is infected with TB, but only 5-10% of those affected go on to develop an active infection—characterized by persistent coughs, fever and chills—and require medical attention. The others remain latent and show no signs of disease. Over the years, several cytokines and other inflammatory signaling molecules have been identified as diagnostic and prognostic markers for TB. An accurate measurement of these biomarkers simultaneously is essential for identifying, distinguishing between and understanding various aspects of latent and active TB infections. A research tool that allows simultaneously measuring these markers will help accelerate TB research and ultimately finding a cure for TB.
The LEGENDplex™ Human Tuberculosis Panel 1 is a bead-based multiplex assay intended for use on various flow cytometers. This panel allows simultaneous quantification of 13 TB markers: VEGF, IL-10, EGF, TNF-α, IL-2, IL-5, GM-CSF, CXCL-10 (IP-10), IFN-γ, IL-1β, IL-6, CXCL-9 (MIG) and TGF-β. This panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant sample.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Tuberculosis Panel 1 Premixed Beads
- LEGENDplex™ Human Tuberculosis Panel 1 Detection Abs
- LEGENDplex™ Human Tuberculosis Panel 1 Std Cktl, Lyoph.
- LEGENDplex™ SA-PE
- LEGENDplex™ Matrix C1, Lyophilized
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- V-bottom Plate
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- Centrifuge with a swinging bucket adaptor for microtiter plates (if
- using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
Antigen Details
- Cell Type
- Dendritic cells, Macrophages, Mesenchymal cells, Neutrophils, NK cells, T cells, Th1
- Biology Area
- Adaptive Immunity, Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 7422 View all products for this Gene ID 3586 View all products for this Gene ID 1950 View all products for this Gene ID 7124 View all products for this Gene ID 3558 View all products for this Gene ID 3567 View all products for this Gene ID 1437 View all products for this Gene ID 3627 View all products for this Gene ID 3458 View all products for this Gene ID 3553 View all products for this Gene ID 3569 View all products for this Gene ID 4283 View all products for this Gene ID 7040 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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