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PBMCs were prepared to isolate CD14+ cells using the MojoSort™ Human CD14 Selection Kit. Cells were stained with CD14 PE (clone HCD14). Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480025 | 20 tests | ¥51,040 | |
| 480026 | 200 tests | ¥162,800 |
CD14+ cells are either selected or depleted by incubating your sample with the biotin conjugated anti-human CD14 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. CD14 is expressed at high levels on human monocytes and macrophages and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cell, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in pathogen clearance and upregulation of adhesion molecules and cytokine expression. Clone 63D3 has been reported not to block LPS induced signal.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480025:
- 1 vial containing 200 µl of biotin anti-human CD14 antibody (clone 63D3)
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat# 480026- 2 vials containing 1 ml each of biotin anti-human CD14 antibody (clone 63D3)
- 2 vials containing 1 ml each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
-
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
- Storage & Handling
- Antibody and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µl of antibody for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer. - Application Notes
-
The expected purity is 90% CD14+ cells post-separation of human peripheral blood mononuclear cells from healthy donors, analyzed by flow cytometry
This kit is designed for the positive selection or depletion of CD14+ cells from peripheral blood mononuclear cells (PBMCs).
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 16X
Nanobead dilution to use in columns: 3X - Product Citations
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Antigen Details
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Gene ID
- NA
- UniProt
- View information about CD14 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
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MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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