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A single cell suspension from adult C57BL/6 brain was prepared using Trypsin digestion and 70/37/30% percoll gradient for positive selection of P2RY12+ cells using the MojoSort™ Mouse P2RY12 Selection Kit. Cells were stained with either anti-mouse P2RY12 (clone S16007D) PE and anti- mouse CD11b (clone M1/70) Brilliant Violet 421™ or anti-mouse CX3CR1 (clone SA011F11) APC. Dead cells were excluded by 7-AAD. -
A single cell suspension from adult C57BL/6 brain was prepared using Trypsin digestion and 70/37/30% percoll gradient for positive selection of P2RY12+ cells using the MojoSort™ Mouse P2RY12 Selection Kit. Cells were stained with either anti-mouse P2RY12 (clone S16007D) PE and anti- mouse CD11b (clone M1/70) Brilliant Violet 421™ or anti-mouse CX3CR1 (clone SA011F11) APC. Dead cells were excluded by 7-AAD.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480113 | 10 tests | ¥53,900 | |
| 480114 | 100 tests | ¥162,800 |
Mouse P2RY12+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse P2RY12 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. Cells collected after magnetic separation can be used for various downstream applications, including functional assays, gene expression analyses, and other phenotypic characterizations.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480113:
- 100 µl of Biotin-Antibody Cocktail: Biotin anti-mouse P2RY12 Antibody (Clone S16007D)
- 100 µl Streptavidin Nanobeads
For Cat# 480114:
- 1 ml of Biotin-Antibody Cocktail: Biotin anti-mouse P2RY12 Antibody (Clone S16007D)
- 1 ml Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
- The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. Conjugated nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µl of antibody cocktail for 1 X 106 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 106 cells in 100 µl of buffer. - Application Notes
-
This kit is designed for the positive selection of mouse microglia cells from neonatal and adult brain.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 40X
Nanobead dilution to use in columns: 12X - Product Citations
-
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- GPCR
- Gene ID
- NA
- UniProt
- View information about P2RY12 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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