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ELISA MAX™ Standard Set Mouse TNF-α

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Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Save
430901 5 plates ¥58,300
Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. Kawamura K,et al.2007.Biol Reprod.76:611.PubMed
  3. Tsujikawa K,et al.2007.Proc Natl Acad Sci USA.104:16702.PubMed
  4. Desy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  5. Sing A, et al. 2011. Infect Immun. 79:4533. PubMed
  6. O'Meara TR, et al. 2013. MBio. 4:e00522. PubMed
  7. Pustelny C, et al. 2015. Infect Immun. 83:417. PubMed
  8. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  9. Maruhashi T, et al. 2015. J Immunol. 194:5681.
Product Citations
  1. Dong L, et al. 2022. Phytother Res. 36:2081. PubMed
  2. Yao R, et al. 2022. Oxid Med Cell Longev. 2022:9469143. PubMed
  3. Rengachar P, et al. 2022. Biomolecules. 12: . PubMed
  4. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  5. Trabjerg MS, et al. 2023. NPJ Parkinsons Dis. 9:6. PubMed
  6. Rengachar P, et al. 2023. Front Endocrinol (Lausanne). 13:1053879. PubMed
  7. Yan K, et al. 2022. Phytother Res. 36:2161. PubMed
  8. Zhao C, et al. 2022. Microbiol Spectr. 10:e0081122. PubMed
  9. Zheng Y, et al. 2022. Int J Mol Sci. 23:. PubMed
  10. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  11. Shao S, et al. 2023. J Mol Med (Berl). 101:419. PubMed
  12. Ge Y, et al. 2023. Front Nutr. 10:1065617. PubMed
  13. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  14. Pang W, et al. 2023. iScience. 26:106579. PubMed
  15. Baek GH, et al. 2023. Nutrients. 15:. PubMed
  16. Pustelny C, et al. 2015. Infect Immun . 83:417. PubMed
  17. Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed
  18. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  19. Swee L, et al. 2016. Open Bio. 6: 160293. PubMed
  20. Poli V, et al. 2021. iScience. 24:103256. PubMed
  21. Zhang W, et al. 2022. Front Pharmacol. 13:1031438. PubMed
  22. Nulty C, et al. 2021. Methods Mol Biol. 2283:175. PubMed
  23. Gomes MT, et al. 2022. J Biol Chem. 298:102034. PubMed
  24. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  25. Rother M, et al. 2018. Cell Host Microbe. 23:661. PubMed
  26. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  27. Aryal P, et al. 2021. J Lipid Res. 62:100108. PubMed
  28. Childers GM, et al. 2021. Environ Health Perspect. 129:47015. PubMed
  29. Renshaw H, et al. 2016. Infect Immun . 84: 1556 - 1564. PubMed
  30. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  31. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  32. Zhao Y, et al. 2018. Mol Cell. 69:505. PubMed
  33. Singh A, et al. 2011. Infect Immun. 79:4533. PubMed
  34. O'Meara T, et al. 2013. MBio. 4:e00522. PubMed
  35. Felgner S, et al. 2020. Elife. 9:00. PubMed
  36. Xu J, et al. 2020. J Am Soc Nephrol. 31:54:00. PubMed
  37. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  38. Fisher AL, et al. 2021. Nat Commun. 12:4026. PubMed
  39. Li F, et al. 2022. Cells. 11:. PubMed
  40. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  41. Vargas-Muñiz J, et al. 2015. Fungal Genet Biol. 81: 41-51. PubMed
  42. Zhou B, et al. 2019. MBio. 10. PubMed
  43. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
  44. Minamidate A, et al. 2021. Biochem Biophys Rep. 27:101065. PubMed
  45. Chen X, et al. 2021. J Extracell Vesicles. 10:e12069. PubMed
  46. Yang J, et al. 2017. Sci Rep. 7:42723. PubMed
  47. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  48. Jung S, et al. 2021. Endocrinology. 162(3):. PubMed
  49. Li HY, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:289. PubMed
  50. Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
  51. Xiao L, et al. 2021. Oxid Med Cell Longev. 2021:6366254. PubMed
  52. Barberi T, et al. 2012. PLoS One. 7:e29808. PubMed
  53. Tsujikawa K, et al. 2007. Proc Natl Acad Sci U S A. 104:16702. PubMed
  54. Chen X, et al. 2019. Mol Pharm. 2.534722222. PubMed
  55. Gu Y, et al. 2021. Front Immunol. 12:635267. PubMed
  56. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  57. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  58. Ho DH, et al. 2018. Exp Neurobiol. 27:171. PubMed
  59. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  60. Liu B, et al. 2021. Theranostics. 11:9311. PubMed
  61. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  62. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
  63. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  64. Frost PS, et al. 2019. Cell Death Dis. 10:323. PubMed
  65. Li X, et al. 2016. J Leukoc Biol. 100: 581 - 588. PubMed
  66. Morzelle M, et al. 2016. PLoS One. 11:e0166123. PubMed
  67. Du H, et al. 2021. Life Sci Alliance. 4: . PubMed
  68. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  69. Du H, et al. 2022. Brain Commun. 11:. PubMed
  70. Li L, et al. 2022. Stem Cell Res Ther. 13:479. PubMed
  71. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  72. Désy O, et al. 2010. Toxicol Sci. 117:303. PubMed
  73. Maruhashi T, et al. 2015. J Immunol. 194:5681. PubMed
  74. Reeves KM, et al. 2021. Clin Cancer Res. Online ahead of print. PubMed
  75. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  76. Kawamura K, et al. 2007. Biol Reprod. 76:611. PubMed
  77. Wong MY, et al. 2020. J Neuroinflammation. 0.841666667. PubMed
  78. Xiong D, et al. 2019. Cell Rep. 28:804. PubMed
RRID
AB_2883995 (BioLegend Cat. No. 430901)
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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