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Normal Serum Block (Previously Covance catalog# SIG-31170)

Pricing & Availability
Regulatory Status
RUO
Other Names
Normal Serum Blocking Solution
Previously
Signet Catalog# 1170
Covance Catalog# SIG-31171
Covance Catalog# SIG-31172
Signet Catalog# 1171
Signet Catalog# 1172
Covance Catalog# SIG-31170
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Product Citations
publications
Normal_Serum_Block_1_HR_090817
Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from rat cerebellum. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3, the tissue was blocked with 5%NGS + 1%BSA (2nd image) or Normal Serum Block (1st image) for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL (diluted in 5%NGS+1%BSA or Normal Serum Block, respectively) overnight at 4°C. Images were captured with a 40X objective (Scale Bar: 20 µm).
  • Normal_Serum_Block_1_HR_090817
    Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from rat cerebellum. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3, the tissue was blocked with 5%NGS + 1%BSA (2nd image) or Normal Serum Block (1st image) for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL (diluted in 5%NGS+1%BSA or Normal Serum Block, respectively) overnight at 4°C. Images were captured with a 40X objective (Scale Bar: 20 µm).
  • Normal_Serum_Block_2_HR_090817
Cat # Size Price Save
927501 6 mL ¥8,580
927502 15 mL ¥12,760
927503 50 mL ¥24,860
Technical data sheet

Product Details

Preparation
Goat Serum
Storage & Handling
Store between 2°C and 8°C.
Application

IHC
Recommended Usage

The optimal working dilution should be determined for each specific assay condition.

Application Notes

Normal goat serum is used as a blocking solution in immunohistochemistry. It is effective in reducing non-specific binding of proteins. For example, Normal Serum Block prevents the secondary antibody from cross reacting with endogenous immunoglobulins in the tissue. The use of normal serum block before the application of primary antibody also eliminates Fc receptor binding of both the primary and secondary antibody.

The Normal Serum Block (cat#s 927501, 927502 ,  927503) is supplied as a blue-colored solution for easy identification when working with multiple IHC reagents. Internal testing has indicated that this product does not interfere with immunofluorescent signals, nor did it contribute to autofluorescence in microscopy work.

Product Citations
  1. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  2. Oravcová M, et al. 2022. Elife. 11:. PubMed
  3. Liu P, et al. 2023. Nat Commun. 6575:14. PubMed
  4. Ghanbari L, et al. 2019. Nat Commun. 10:1500. PubMed
  5. Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed
  6. Prasad S, et al. 2020. Commun Biol. 0.675. PubMed
  7. Nie M, et al. 2021. EMBO Rep. 22:e50803. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 2    Revision Date: 11/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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