Pacific Blue anti-mouse CD45RB Antibody anti-CD45RB

Clone: C363-16A (See other available formats)
Regulatory Status: RUO
Other Names: CD45RB
Isotype: Rat IgG2a, κ
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C363-16A_PB_040810 — C57BL/6 splenocytes stained with C363-16A Pacific Blue™

Compare all formats See Pacific Blue™ spectral data

Cat #	Size	Price	Save
103315	25 µg	¥28,380
103316	100 µg	¥71,060

Description

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4⁺ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Cloned mouse Th2 cell lines
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
RRID: AB_2174405 (BioLegend Cat. No. 103315)
AB_2174405 (BioLegend Cat. No. 103316)

Antigen Details

Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells
Function: Phosphatase, T cell development and activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, Thy1
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45RB on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD45RB Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD45RB	C363-16A	FC
PE anti-mouse CD45RB	C363-16A	FC
Purified anti-mouse CD45RB	C363-16A	FC,IHC-P,IHC-F,IP
APC/Cyanine7 anti-mouse CD45RB	C363-16A	FC
Alexa Fluor® 647 anti-mouse CD45RB	C363-16A	FC
PerCP/Cyanine5.5 anti-mouse CD45RB	C363-16A	FC
Pacific Blue™ anti-mouse CD45RB	C363-16A	FC
PE/Cyanine7 anti-mouse CD45RB	C363-16A	FC
APC anti-mouse CD45RB	C363-16A	FC
TotalSeq™-C1063 anti-mouse CD45RB Antibody	C363-16A	PG
Biotin anti-mouse CD45RB	C363-16A	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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