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Pacific Blue™ anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
53-6dot7_Blue_091207
C57BL/6 mouse splenocytes were stained with CD8 (clone 53-6.7) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
  • 53-6dot7_Blue_091207
    C57BL/6 mouse splenocytes were stained with CD8 (clone 53-6.7) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Save
100728 25 µg ¥16,740
100725 100 µg ¥38,270
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  2. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  3. Chen Y, et al. 2020. Virol Sin. . PubMed
  4. Ramos SA, et al. 2022. J Allergy Clin Immunol. 149:767. PubMed
  5. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  6. Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed
  7. Balood M, et al. 2022. Nature. 611:405. PubMed
  8. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  9. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  10. Novaj A, et al. 2023. Int J Mol Sci. 24: . PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  13. Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed
  14. Olson SW, et al. 2022. Mol Cell. 82:1708. PubMed
  15. McCafferty S, et al. 2022. Mol Ther. 30:2968. PubMed
  16. Dhalech AH, et al. 2022. J Virol. 96:e0123222. PubMed
  17. Ke F, et al. 2023. Elife. 12:. PubMed
  18. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  19. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  20. Gawish R, et al. 2022. Elife. 11:. PubMed
  21. Zhu W, et al. 2021. Onco Targets Ther. 14:2149. PubMed
  22. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  23. Hamilton JAG, et al. 2021. Aging Cell. 20:e13309. PubMed
  24. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  25. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  26. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  27. Schneider C, et al. 2018. Cell. 174:271. PubMed
  28. Page N, et al. 2018. Immunity. 48:937. PubMed
  29. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  30. Engler J, et al. 2017. Proc Natl Acad Sci U S A. 114(2):E181-E190. PubMed
  31. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  32. Gu M, et al. 2021. Nat Immunol. 22:193. PubMed
  33. Galeano Niño JL, et al. 2020. J Cell Sci. . PubMed
  34. Saito S, et al. 2020. Nutrients. 12:. PubMed
  35. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  36. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  37. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  38. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  39. Price J, et al. 2015. Diabetes. 64: 3521 - 3531. PubMed
  40. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  41. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  42. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  43. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  44. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  45. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  46. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  47. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  48. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  49. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  50. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  51. Atkinson S, et al. 2016. Dis Model Mech. 9: 427-440. PubMed
  52. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  53. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  54. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  55. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  56. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  57. Galeano Niño JL, et al. 2020. J Cell Sci. 133:00:00. PubMed
  58. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  59. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  60. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  61. Chun N, et al. 2021. JCI Insight. 6:. PubMed
  62. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  63. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  64. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  65. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  66. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  67. López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed
  68. Mohebiany AN, et al. 2020. Cell Reports. 30(5):1585-1597.e6.. PubMed
  69. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  70. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  71. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  72. Tian Q, et al. 2022. Front Mol Neurosci. 15:850904. PubMed
  73. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  74. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  75. Engblom C, et al. 2017. Science. 358:6367. PubMed
  76. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  77. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  78. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  79. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  80. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  81. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  82. Zhang Z, et al. 2020. Nature. 579:415. PubMed
  83. Wang X, et al. 2022. Elife. 11:. PubMed
  84. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  85. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  86. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  87. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  88. Chen D, et al. 2021. JCI Insight. 6:. PubMed
  89. Gawish R, et al. 2022. Elife. 11:. PubMed
  90. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
  91. Wolf Y, et al. 2019. Cell. 179:219. PubMed
RRID
AB_493425 (BioLegend Cat. No. 100728)
AB_493425 (BioLegend Cat. No. 100725)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
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For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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